Treffer: An antigen microarray protocol for COVID-19 serological analysis.
Title:
An antigen microarray protocol for COVID-19 serological analysis.
Authors:
Source:
STAR Protocols, 2 (3), 100815 (2021-09-17)
Publisher Information:
Cell Press
Publication Year:
2021
Collection:
University of Luxembourg: ORBilu - Open Repository and Bibliography
Subject Terms:
Antibody, Bioinformatics, Health Sciences, High Throughput Screening, Immunology, Proteomics, Antibodies, Viral, Antigens, Spike Glycoprotein, Coronavirus, Viral/blood, Viral/immunology, COVID-19/blood, COVID-19/immunology, COVID-19/virology, Humans, Microarray Analysis/methods, SARS-CoV-2/immunology, Coronavirus/blood, Coronavirus/immunology, COVID-19, Microarray Analysis, SARS-CoV-2, Biochemistry, Genetics and Molecular Biology (all), Neuroscience (all), Immunology and Microbiology (all), Medicine (all), General Immunology and Microbiology
Document Type:
Fachzeitschrift
article in journal/newspaper
Language:
English
ISSN:
2666-1667
Relation:
https://api.elsevier.com/content/article/PII:S2666166721005219?httpAccept=text/xml; urn:issn:2666-1667; https://orbilu.uni.lu/handle/10993/58213; info:hdl:10993/58213; info:pmid:34462737; wos:001048876800005
DOI:
10.1016/j.xpro.2021.100815
Availability:
Rights:
open access ; http://purl.org/coar/access_right/c_abf2 ; info:eu-repo/semantics/openAccess
Accession Number:
edsbas.DF81755F
Database:
BASE
Weitere Informationen
peer reviewed ; The emergence of the coronavirus disease 2019 pandemic increased the interest in analysis of immunoglobulin responses. ELISA and lateral flow assays are widely used but are restricted by a single response value to an antigen or antigen pool. Here, we describe antigen microarrays, an alternative allowing simultaneous assessment of multiple interactions between antigens and the immunoglobulin content of patient sera. The technique requires minimal reagents and sample input and can be adapted to a wide variety of potential antigenic targets of interest.