Treffer: Fast structured illumination for 3-D high-resolution fluorescent imaging

Title:
Fast structured illumination for 3-D high-resolution fluorescent imaging
Contributors:
Meng, Yunlong (author.), Chen, Shih-chi , 1977- (thesis advisor.), Chinese University of Hong Kong Graduate School. Division of Mechanical and Automation Engineering. (degree granting institution.)
Publication Year:
2019
Collection:
The Chinese University of Hong Kong: CUHK Digital Repository / 香港中文大學數碼典藏
Subject Terms:
Image processing--Mathematics, Image reconstruction--Mathematics, Optical data processing, Diagnostic imaging--Data processing, Imaging systems in medicine, Three-dimensional imaging in medicine, Image Enhancement--methods, Image Processing, Computer-Assisted--methods, Optical Imaging, Imaging, Three-Dimensional, Algorithms
Document Type:
Fachzeitschrift text
File Description:
electronic resource; remote; 1 online resource (i leaf, 5 unnumbered leaves, ii-xiii, 148 leaves) : illustrations (chiefly color); computer; online resource
Language:
English
Chinese
Relation:
cuhk:2327253; local: ETD920200909; local: 991039842704803407
Availability:
https://julac.hosted.exlibrisgroup.com/primo-explore/search?query=addsrcrid,exact,991039842704803407,AND&tab=default_tab&search_scope=All&vid=CUHK&mode=advanced&lang=en_US
https://repository.lib.cuhk.edu.hk/en/item/cuhk-2327253
Rights:
Use of this resource is governed by the terms and conditions of the Creative Commons "Attribution-NonCommercial-NoDerivatives 4.0 International" License (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Accession Number:
edsbas.59813FB3
Database:
BASE

Weitere Informationen

Ph.D. ; The aim of this research is to develop new structured light illumination (SLI) reconstruction methods and algorithms for fast data acquisition and fluorescent imaging with improved signal-to-noise ratio (SNR) and 3-D imaging resolution. Specially, a new two-snapshot SLI algorithm is developed for improving the axial resolution of wide-field multi-photon imaging systems; and a five-snapshot SLI algorithm is developed to achieve in-plane super-resolution imaging for single-photon wide-field imaging systems. The new algorithms have been experimentally verified via imaging experiments on biological samples. ; The fast two-snapshot SLI algorithm, which only requires two mutually π phase-shifted raw structured images, is realized via a unique parallel amplitude demodulation method, derived based on 1D discrete Hilbert transform. The new algorithm is implemented on a custom-built multi-photon temporal focusing microscopy (TFM), where a DMD replaces the generic blazed grating in the system and simultaneously functions as a diffraction grating and a programmable binary mask, generating arbitrary fringe patterns. The experimental results show promising depth-discrimination capability with an axial resolution enhancement rate of 24.6%, which matches well with the theoretical estimation, i.e, 27.0%. Imaging experiments indicate that the two-snapshot algorithm presents comparable optical cross-sectioning capability and better contrast reconstruction and SNR than those adopting conventional root-mean-square (RMS) reconstruction method which require five images. The five-snapshot super-resolution SLI algorithm is inspired from the Fourier ptychography algorithm, which includes new methods for localizing the frequency vectors, determining phase shifts, and evaluating the modulation. Overall, the five snapshot super-resolution SLI algorithm improves the lateral imaging resolution by a factor of 2.02 (~3% deviation from theory), while simultaneously improving the imaging speed by a factor of 1.8 when comparing with the ...