• Comparative Gene Expression An...

Treffer: Comparative Gene Expression Analysis of the Coronal Pulp and Apical Pulp Complex in Human Immature Teeth

Title:
Comparative Gene Expression Analysis of the Coronal Pulp and Apical Pulp Complex in Human Immature Teeth
Contributors:
College of Dentistry, Dept. of Pediatric Dentistry, Soo-Hyun Kim, Seunghye Kim, Yooseok Shin, Hyo-Seol Lee, Mijeong Jeon, Seong-Oh Kim, Sung-Won Cho, Nikita B. Ruparel, Je Seon Song, Kim, Seong Oh, Song, Je Seon, Shin, Yoo Seok, Cho, Sung Won
Publisher Information:
Elsevier
United States
Publication Year:
2016
Subject Terms:
Adolescent, CX3C Chemokine Receptor 1, Calbindin 1/genetics, Calcium-Binding Proteins/genetics, Cell Adhesion/genetics, Child, Preschool, Collagen Type XII/genetics, Dental Pulp/anatomy & histology, Dental Pulp/cytology, Dental Pulp/diagnostic imaging, Dental Pulp/growth & development, Extracellular Matrix Proteins/genetics, Female, Gene Expression, Gene Expression Profiling, Hedgehog Proteins/genetics, Homeodomain Proteins/genetics, Humans, Immunohistochemistry, Laminin/genetics, Male, Microarray Analysis/methods, Neurogenesis/genetics, Odontogenesis/genetics, Osteopontin/genetics, Phosphoproteins/genetics, RNA/analysis, Real-Time Polymerase Chain Reaction/methods, Receptors
Document Type:
Fachzeitschrift article in journal/newspaper
File Description:
752~759
Language:
English
Relation:
JOURNAL OF ENDODONTICS; J01393; https://ir.ymlib.yonsei.ac.kr/handle/22282913/146792; http://www.sciencedirect.com/science/article/pii/S0099239916000959; T201601319; 47534
DOI:
10.1016/j.joen.2016.01.024
Availability:
https://ir.ymlib.yonsei.ac.kr/handle/22282913/146792
https://doi.org/10.1016/j.joen.2016.01.024
http://www.sciencedirect.com/science/article/pii/S0099239916000959
Rights:
CC BY-NC-ND 2.0 KR ; https://creativecommons.org/licenses/by-nc-nd/2.0/kr/
Accession Number:
edsbas.12FD18D4
Database:
BASE

Weitere Informationen

INTRODUCTION: This study determined the gene expression profiles of the human coronal pulp (CP) and apical pulp complex (APC) with the aim of explaining differences in their functions. METHODS: Total RNA was isolated from the CP and APC, and gene expression was analyzed using complementary DNA microarray technology. Gene ontology analysis was used to classify the biological function. Quantitative reverse-transcription polymerase chain reaction and immunohistochemical staining were performed to verify microarray data. RESULTS: In the microarray analyses, expression increases of at least 2-fold were present in 125 genes in the APC and 139 genes in the CP out of a total of 33,297 genes. Gene ontology class processes found more genes related to immune responses, cell growth and maintenance, and cell adhesion in the APC, whereas transport and neurogenesis genes predominated in the CP. Quantitative reverse-transcription polymerase chain reaction and immunohistochemical staining confirmed the microarray results, with DMP1, CALB1, and GABRB1 strongly expressed in the CP, whereas SMOC2, SHH, BARX1, CX3CR1, SPP1, COL XII, and LAMC2 were strongly expressed in the APC. CONCLUSIONS: The expression levels of genes related to dentin mineralization, neurogenesis, and neurotransmission are higher in the CP in human immature teeth, whereas those of immune-related and tooth development-related genes are higher in the APC. ; restriction