Treffer: Solid Medium Droplet Microarray for Miniaturized Antimicrobial Susceptibility Test.

Antimicrobial susceptibility testing (AST) that is easily adaptable for point‐of‐care (POC) use is essential for addressing the growing threat of antibiotic resistance. Here, the solid medium droplet microarray (SM‐DMA), a simple yet versatile testing platform consisting of a single microscope slide patterned with an array of 80 agar droplets (6–8 μL each), containing customizable combinations of clinically relevant antibiotics, is introduced. The test allows for easy manual sample application and features a colorimetric self‐check readout. Using E. coli (DSM498) as a model organism, accurate determination of minimum inhibitory concentrations for clinically relevant antibiotics (cefotaxime, ciprofloxacin, and ampicillin), producing results consistent with EUCAST clinical breakpoints, is demonstrated. Furthermore, SM‐DMA facilitates combinatorial antibiotic testing, represented by intuitive viability heatmaps. The platform is more time efficient (≈16–18 h total) compared to the conventional agar plate‐based methods. Owing to the robustness, ease of use, and independence from specialized equipment, the SM‐DMA can be adapted for POC applications by nontrained personnel or even by patients themselves. [ABSTRACT FROM AUTHOR]

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