• The synergistic effect of phot...

Treffer: The synergistic effect of photobiomodulation, methylglyoxal, and complex magnetic fields on human dermal fibroblasts: potential applications for chronic wound treatments.

Title:
The synergistic effect of photobiomodulation, methylglyoxal, and complex magnetic fields on human dermal fibroblasts: potential applications for chronic wound treatments.
Authors:
D'Amico E; Department of Medical, Oral and Biotechnological Science, University of Chieti-Pescara, Chieti, Italy., Pierfelice TV; Department of Medical, Oral and Biotechnological Science, University of Chieti-Pescara, Chieti, Italy. tania.pierfelice@unich.it., D'Ercole L; Department of Medical Physics, Fondazione IRCCS Policlinico San Matteo, 27100, Pavia, Italy., Di Fermo P; Department of Medical, Oral and Biotechnological Science, University of Chieti-Pescara, Chieti, Italy., Iezzi G; Department of Medical, Oral and Biotechnological Science, University of Chieti-Pescara, Chieti, Italy., D'ercole S; Department of Medical, Oral and Biotechnological Science, University of Chieti-Pescara, Chieti, Italy., Petrini M; Department of Medical, Oral and Biotechnological Science, University of Chieti-Pescara, Chieti, Italy. morena.petrini@unich.it.
Source:
Lasers in medical science [Lasers Med Sci] 2025 Dec 11; Vol. 40 (1), pp. 517. Date of Electronic Publication: 2025 Dec 11.
Publication Type:
Journal Article
Language:
English
Journal Info:
Publisher: Springer Country of Publication: England NLM ID: 8611515 Publication Model: Electronic Cited Medium: Internet ISSN: 1435-604X (Electronic) Linking ISSN: 02688921 NLM ISO Abbreviation: Lasers Med Sci Subsets: MEDLINE
Imprint Name(s):
Publication: London : Springer
Original Publication: London : Baillière Tindall, c1986-
MeSH Terms:
Fibroblasts*/drug effects , Fibroblasts*/radiation effects , Pyruvaldehyde*/pharmacology , Pyruvaldehyde*/therapeutic use , Low-Level Light Therapy*/methods , Wound Healing*/drug effects , Wound Healing*/radiation effects , Magnetic Fields* , Magnetic Field Therapy*, Humans ; Cell Survival/drug effects ; Cell Survival/radiation effects ; Cell Movement/drug effects ; Cell Movement/radiation effects ; Cells, Cultured ; Collagen Type I/metabolism ; Collagen Type I/biosynthesis ; Combined Modality Therapy
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Contributed Indexing:
Keywords: Derma; MGO; Magnetic fields; Photobiomodulation; Regeneration
Substance Nomenclature:
722KLD7415 (Pyruvaldehyde)
0 (Collagen Type I)
Entry Date(s):
Date Created: 20251210 Date Completed: 20251211 Latest Revision: 20251213
Update Code:
20251213
PubMed Central ID:
PMC12695920
DOI:
10.1007/s10103-025-04775-3
PMID:
41372538
Database:
MEDLINE

Weitere Informationen

This paper aimed to verify how a new protocol, recently proposed for treating chronic wounds due to its excellent antimicrobial properties, affects human dermal fibroblasts (NHDFs). Single and combined action of light-emitting diodes (LED), complex magnetic fields (CMFs), and methylglyoxal (MGO) on cell viability and activity of NHDFs were investigated. Our first objective was to exclude any toxicity of this combined treatment on these cells. NHDFs were exposed to LED light for 17 min, CMFs for 22 min, MGO, MGO + LED, and MGO + CMFs, and then were assessed for cell viability, morphology, cytoskeletal integrity, collagen type I production, and migration capacity. Results of combined treatments were compared with those of single treatments and unexposed controls. NHDFs exposed to both single and combined treatments maintained viability, morphology, and cytoskeletal integrity, showing no signs of cytotoxicity. MGO at low concentrations was non-toxic and, when combined with other technologies, could confer beneficial effects on cell adhesion. LED stimulated collagen type I synthesis, and the production increased in samples subjected to the combined action of MGO + LED. CMFs notably accelerated fibroblasts' migration in scratch assays, and when combined with MGO, they further enhanced this effect. The effects of MGO + LED and MGO + CMFs were probably due to cellular uptake and receptor sensitivity. The tested protocols were not only non-toxic but also promoted beneficial effects on the vitality and activity of dermal fibroblasts, confirming their potential in treating chronic wounds.
(© 2025. The Author(s).)

Declarations. Competing interests: The authors declare no competing interests. The funders were not involved in the study design; in the collection, analysis, and interpretation of data; in the writing of this article; or in the decision to submit it for publication.