• Proteases in bacteriophage def...

Treffer: Proteases in bacteriophage defense systems and their potential in bioengineering.

Title:
Proteases in bacteriophage defense systems and their potential in bioengineering.
Authors:
Kalogeropoulos K; Department of Bionanoscience, Delft University of Technology, 2629 HZ Delft, The Netherlands; Kavli Institute of Nanoscience, 2629 HZ Delft, The Netherlands; Department of Biotechnology and Biomedicine, Technical University of Denmark, Søltofts Plads B221, Kgs. Lyngby, 2800, Denmark. Electronic address: konka@dtu.dk., van Beljouw SPB; Department of Bionanoscience, Delft University of Technology, 2629 HZ Delft, The Netherlands; Kavli Institute of Nanoscience, 2629 HZ Delft, The Netherlands., Feldmann D; Department of Bionanoscience, Delft University of Technology, 2629 HZ Delft, The Netherlands; Kavli Institute of Nanoscience, 2629 HZ Delft, The Netherlands., van den Berg DF; Department of Bionanoscience, Delft University of Technology, 2629 HZ Delft, The Netherlands; Kavli Institute of Nanoscience, 2629 HZ Delft, The Netherlands., Brouns SJJ; Department of Bionanoscience, Delft University of Technology, 2629 HZ Delft, The Netherlands; Kavli Institute of Nanoscience, 2629 HZ Delft, The Netherlands. Electronic address: stanbrouns@gmail.com.
Source:
Trends in biochemical sciences [Trends Biochem Sci] 2026 Jan; Vol. 51 (1), pp. 64-79. Date of Electronic Publication: 2025 Nov 15.
Publication Type:
Journal Article; Review
Language:
English
Journal Info:
Publisher: Elsevier Trends Journals Country of Publication: England NLM ID: 7610674 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 0968-0004 (Print) Linking ISSN: 09680004 NLM ISO Abbreviation: Trends Biochem Sci Subsets: MEDLINE
Imprint Name(s):
Publication: Cambridge (Eng) : Elsevier Trends Journals
Original Publication: Amsterdam, Published for the International Union of Biochemistry by Elsevier Scientific Pub. Co.
MeSH Terms:
Bacteriophages*/enzymology , Bacteriophages*/metabolism , Bioengineering*/methods , Peptide Hydrolases*/metabolism
Contributed Indexing:
Keywords: CRISPR–Cas; phage defense; protease effectors; protease-associated defense systems (PADS); protein engineering; proteome editing
Substance Nomenclature:
EC 3.4.- (Peptide Hydrolases)
Entry Date(s):
Date Created: 20251115 Date Completed: 20260109 Latest Revision: 20260110
Update Code:
20260111
DOI:
10.1016/j.tibs.2025.10.006
PMID:
41241617
Database:
MEDLINE

Weitere Informationen

Novel phage defense systems featuring diverse enzymatic activities are continually being discovered. Among these, defense systems employing proteolytic enzymes have been identified, revealing a previously unrecognized enzymatic activity in phage defense. These protease-associated defense systems represent an untapped reservoir for new biotechnological tools and may serve as a springboard for the development of proteome editors. This review outlines recent advancements in the discovery and characterization of protease-containing defense systems, proposes methods for further exploration and investigation of protease activity, and considers the prospect of protease defense systems for modulating protein processing and cell fate.
(Copyright © 2025 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Declaration of interests The authors declare no conflicts of interest.