• Flavin Biosynthesis Enhances E...

Treffer: Flavin Biosynthesis Enhances Extracellular Electron Transfer in Bioengineered Escherichia coli.

Title:
Flavin Biosynthesis Enhances Extracellular Electron Transfer in Bioengineered Escherichia coli.
Authors:
Mouhib M; Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, 1015, Switzerland., Reggente M; Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, 1015, Switzerland., Wang H; Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, 1015, Switzerland., Roullier C; Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, 1015, Switzerland., Boghossian AA; Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, 1015, Switzerland.
Source:
Advanced science (Weinheim, Baden-Wurttemberg, Germany) [Adv Sci (Weinh)] 2026 Jan; Vol. 13 (2), pp. e2412230. Date of Electronic Publication: 2025 Nov 01.
Publication Type:
Journal Article
Language:
English
Journal Info:
Publisher: WILEY-VCH Country of Publication: Germany NLM ID: 101664569 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2198-3844 (Electronic) Linking ISSN: 21983844 NLM ISO Abbreviation: Adv Sci (Weinh) Subsets: MEDLINE
Imprint Name(s):
Original Publication: Weinheim : WILEY-VCH, [2014]-
MeSH Terms:
Escherichia coli*/metabolism , Escherichia coli*/genetics , Flavins*/biosynthesis , Flavins*/metabolism , Bioengineering*/methods, Shewanella/metabolism ; Shewanella/genetics ; Electron Transport/physiology ; Riboflavin/metabolism ; Flavin Mononucleotide/metabolism ; Flavin Mononucleotide/biosynthesis
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Grant Information:
GRS-074/17 Gebert Rüf Stiftung; 501100001711-154269 Switzerland SNSF_ Swiss National Science Foundation
Contributed Indexing:
Keywords: bioelectrochemistry; extracellular electron transfer; microbial fuel cell; redox mediator; synthetic biology
Substance Nomenclature:
0 (Flavins)
TLM2976OFR (Riboflavin)
7N464URE7E (Flavin Mononucleotide)
SCR Organism:
Shewanella oneidensis
Entry Date(s):
Date Created: 20251101 Date Completed: 20260109 Latest Revision: 20260111
Update Code:
20260111
PubMed Central ID:
PMC12786374
DOI:
10.1002/advs.202412230
PMID:
41176478
Database:
MEDLINE

Weitere Informationen

Advancements in bioengineering have unlocked new microbial electrochemical applications in energy, sensing, remediation, and synthesis. Key to realizing these technologies is the engineering of conduits in metabolically versatile microbes like Escherichia coli to enable efficient charge exchange with the electrode. Inspired by mechanisms found in natural exogelectrogens, previous studies have largely focused on introducing conduits based on the metal-reducing (Mtr) pathway in Shewanella oneidensis MR-1. This study explores the concomitant expression of flavin secretion pathways for mediated charge transfer to complement the direct charge transfer from the bioengineered Mtr pathway. The engineered strains show a 3-fold increase in the total secretion of flavin mononucleotide (FMN) and riboflavin compared to a state-of-the-art Mtr-expressing strain lacking flavin overexpression. The concomitant flavin secretion further contributes up to a ≈3.4- and ≈1.5-fold increase in current compared to unmodified cells and the previous Mtr-expressing cells, respectively, with the greatest currents achieved for the strain favoring riboflavin secretion over FMN secretion. The introduction of flavin biosynthesis genes to Mtr-expressing strains thus reveals a distinct, yet complementary, EET mechanism for robust and multi-modal microbial applications.
(© 2025 The Author(s). Advanced Science published by Wiley‐VCH GmbH.)