Treffer: Protocol for decellularizing and delipidizing human pancreatic tissue into native hydrogel.

Title:
Protocol for decellularizing and delipidizing human pancreatic tissue into native hydrogel.
Authors:
Chamberlain CS; Division of Transplantation, Department of Surgery, University of Wisconsin-Madison, Madison, WI 53705, USA., Tremmel DM; Division of Transplantation, Department of Surgery, University of Wisconsin-Madison, Madison, WI 53705, USA. Electronic address: daniel.tremmel@childrens.harvard.edu., Khan AP; Division of Transplantation, Department of Surgery, University of Wisconsin-Madison, Madison, WI 53705, USA., Santos EA; Division of Transplantation, Department of Surgery, University of Wisconsin-Madison, Madison, WI 53705, USA., Kapadia D; Division of Transplantation, Department of Surgery, University of Wisconsin-Madison, Madison, WI 53705, USA., Leavens C; Division of Transplantation, Department of Surgery, University of Wisconsin-Madison, Madison, WI 53705, USA., Gorski KM; Division of Transplantation, Department of Surgery, University of Wisconsin-Madison, Madison, WI 53705, USA., Hamadeh R; Division of Transplantation, Department of Surgery, University of Wisconsin-Madison, Madison, WI 53705, USA., Feeney AK; Division of Transplantation, Department of Surgery, University of Wisconsin-Madison, Madison, WI 53705, USA., Odorico J; Division of Transplantation, Department of Surgery, University of Wisconsin-Madison, Madison, WI 53705, USA. Electronic address: jon@surgery.wisc.edu., Sackett SD; Division of Transplantation, Department of Surgery, University of Wisconsin-Madison, Madison, WI 53705, USA. Electronic address: sara.duttonsackett@pennmedicine.upenn.edu.
Source:
STAR protocols [STAR Protoc] 2025 Sep 19; Vol. 6 (3), pp. 103997. Date of Electronic Publication: 2025 Jul 29.
Publication Type:
Journal Article
Language:
English
Journal Info:
Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE
Imprint Name(s):
Original Publication: [Cambridge, MA] : Cell Press, [2020]-
MeSH Terms:
Pancreas*/cytology , Pancreas*/chemistry , Hydrogels*/chemistry , Decellularized Extracellular Matrix*/chemistry , Tissue Engineering*/methods, Humans ; Extracellular Matrix/chemistry ; Tissue Scaffolds/chemistry
Contributed Indexing:
Keywords: Biotechnology and bioengineering; Organoids; Tissue Engineering
Substance Nomenclature:
0 (Hydrogels)
0 (Decellularized Extracellular Matrix)
Entry Date(s):
Date Created: 20250731 Date Completed: 20250923 Latest Revision: 20250923
Update Code:
20250923
PubMed Central ID:
PMC12332197
DOI:
10.1016/j.xpro.2025.103997
PMID:
40742816
Database:
MEDLINE

Weitere Informationen

We present a protocol for transforming human pancreatic tissue into a decellularized and delipidized extracellular matrix (ECM) for hydrogel/scaffold applications. We describe steps for decellularization and delipidization using sodium deoxycholate and sequential ethanol, acetone, and hexane washes and removal of nucleic acids with benzonase. We then detail procedures for lyophilizing the ECM for long-term preservation and digesting the pancreatic ECM in pepsin-HCL to form a hydrogel. For complete details on the use and execution of this protocol, please refer to Tremmel et al. <sup>1</sup> and Sackett et al. <sup>2</sup> .
(Copyright © 2025 The Author(s). Published by Elsevier Inc. All rights reserved.)

Declaration of interests J.O. is a scientific cofounder of Regenerative Medical Solutions, Inc., and has stock equity.