• Protocol for the fabrication o...

Treffer: Protocol for the fabrication of self-standing (nano)cellulose-based 3D scaffolds for tissue engineering.

Title:
Protocol for the fabrication of self-standing (nano)cellulose-based 3D scaffolds for tissue engineering.
Authors:
Mohan T; Graz University of Technology, Institute for Chemistry and Technology of Biobased System (IBioSys), Stremayrgasse 9, 8010 Graz, Austria; University of Maribor, Faculty of Mechanical Engineering, Laboratory for Characterization and Processing of Polymers, Smetanova Ulica 17, 2000 Maribor, Slovenia. Electronic address: tamilselvan.mohan@tugraz.at., Bračič M; University of Maribor, Faculty of Mechanical Engineering, Laboratory for Characterization and Processing of Polymers, Smetanova Ulica 17, 2000 Maribor, Slovenia. Electronic address: matej.bracic@um.si., Bračič D; University of Maribor, Faculty of Mechanical Engineering, Laboratory for Characterization and Processing of Polymers, Smetanova Ulica 17, 2000 Maribor, Slovenia., Lackner F; Graz University of Technology, Institute for Chemistry and Technology of Biobased System (IBioSys), Stremayrgasse 9, 8010 Graz, Austria; Members of the European Polysaccharide Network of Excellence (EPNOE)., Nagaraj C; Department of Internal Medicine, Division of Pulmonology, Medical University of Graz, Graz, Austria., Štiglic AD; University of Maribor, Faculty of Mechanical Engineering, Laboratory for Characterization and Processing of Polymers, Smetanova Ulica 17, 2000 Maribor, Slovenia., Kargl R; Graz University of Technology, Institute for Chemistry and Technology of Biobased System (IBioSys), Stremayrgasse 9, 8010 Graz, Austria; University of Maribor, Faculty of Mechanical Engineering, Laboratory for Characterization and Processing of Polymers, Smetanova Ulica 17, 2000 Maribor, Slovenia; Members of the European Polysaccharide Network of Excellence (EPNOE)., Kleinschek KS; Graz University of Technology, Institute for Chemistry and Technology of Biobased System (IBioSys), Stremayrgasse 9, 8010 Graz, Austria; Institute of Automation, Faculty of Electrical Engineering and Computer Science, University of Maribor, Maribor, Slovenia; Members of the European Polysaccharide Network of Excellence (EPNOE).
Source:
STAR protocols [STAR Protoc] 2025 Mar 21; Vol. 6 (1), pp. 103583. Date of Electronic Publication: 2025 Jan 24.
Publication Type:
Journal Article
Language:
English
Journal Info:
Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE
Imprint Name(s):
Original Publication: [Cambridge, MA] : Cell Press, [2020]-
MeSH Terms:
Tissue Engineering*/methods , Cellulose*/chemistry , Tissue Scaffolds*/chemistry , Nanostructures*/chemistry, Humans ; Printing, Three-Dimensional ; Biocompatible Materials/chemistry ; Skin/cytology
Contributed Indexing:
Keywords: Biotechnology and bioengineering; Chemistry; Material sciences; Tissue Engineering
Substance Nomenclature:
9004-34-6 (Cellulose)
0 (Biocompatible Materials)
Entry Date(s):
Date Created: 20250125 Date Completed: 20250503 Latest Revision: 20250503
Update Code:
20250505
PubMed Central ID:
PMC11803828
DOI:
10.1016/j.xpro.2024.103583
PMID:
39862432
Database:
MEDLINE

Weitere Informationen

Three-dimensional (3D) and porous scaffolds made from nanocellulosic materials hold significant potential in tissue engineering (TE). Here, we present a protocol for fabricating self-standing (nano)cellulose-based 3D scaffolds designed for in vitro testing of cells from skin and cartilage tissues. We describe steps for preparation of nanocellulose ink, scaffold formation using 3D printing, and freeze-drying. We then detail post-processing procedures to enhance mechanical properties, stability, and biocompatibility. This protocol offers researchers a framework for developing versatile and sustainable biomaterials for regenerative medicine. For complete details on the use and execution of this protocol, please refer to Mohan et al. <sup>1</sup> and Štiglic et al. <sup>2</sup> .
(Copyright © 2025 The Authors. Published by Elsevier Inc. All rights reserved.)

Declaration of interests The authors declare no competing interests.