• Protocol for genetic engineeri...

Treffer: Protocol for genetic engineering in Drosophila suzukii using microinjection.

Title:
Protocol for genetic engineering in Drosophila suzukii using microinjection.
Authors:
Yan Y; Institute for Insect Biotechnology, Department of Insect Biotechnology in Plant Protection, Justus-Liebig-University Gießen, Winchesterstraße 2, 35394 Gießen, Germany. Electronic address: ying.yan@agrar.uni-giessen.de., Schetelig MF; Institute for Insect Biotechnology, Department of Insect Biotechnology in Plant Protection, Justus-Liebig-University Gießen, Winchesterstraße 2, 35394 Gießen, Germany. Electronic address: marc.schetelig@agrar.uni-giessen.de.
Source:
STAR protocols [STAR Protoc] 2024 Sep 20; Vol. 5 (3), pp. 103248. Date of Electronic Publication: 2024 Aug 14.
Publication Type:
Journal Article; Research Support, Non-U.S. Gov't
Language:
English
Journal Info:
Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE
Imprint Name(s):
Original Publication: [Cambridge, MA] : Cell Press, [2020]-
MeSH Terms:
Drosophila*/genetics , Microinjections*/methods , Genetic Engineering*/methods , Gene Editing*/methods, Animals ; CRISPR-Cas Systems/genetics ; DNA Transposable Elements/genetics ; Gene Targeting/methods
Contributed Indexing:
Keywords: Biotechnology and bioengineering; CRISPR; Genetics; Molecular Biology
Substance Nomenclature:
0 (DNA Transposable Elements)
Entry Date(s):
Date Created: 20240815 Date Completed: 20240926 Latest Revision: 20250610
Update Code:
20250611
PubMed Central ID:
PMC11372800
DOI:
10.1016/j.xpro.2024.103248
PMID:
39146186
Database:
MEDLINE

Weitere Informationen

The spotted wing Drosophila (Drosophila suzukii Matsumura) is recognized globally as a significant economic pest. Here, we present a protocol for genetic engineering in D. suzukii using microinjection. We describe steps for genetic engineering techniques, including transposon-mediated germline transformation, recombinase-mediated genome targeting, and CRISPR-mediated gene editing. This protocol can significantly expand the toolkit for functional genomics and genetic control studies of this pest. For complete details on the use and execution of this protocol, please refer to Schetelig and Handler, <sup>1</sup> Schetelig et al. <sup>2</sup> Yan et al., <sup>3</sup> and Yan et al. <sup>4</sup> .
(Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)

Declaration of interests The authors declare no competing interests.